Adaptive PCR: A PCR control system to overcome challenging conditions

Applications

By directly monitoring the hybridization of fluorescent-labeled L-DNA mimics of the template DNA strands and primers, it is possible to improve the efficiency of PCR in challenging conditions. This approach eliminates some of the sample preparation and trial and error that would otherwise be required for difficult sample types such as urine or other samples that contain high levels of salts.  In addition, this approach enables on-demand PCR in most any environment, such as:

• At the point-of-care in a physician’s office or pharmacy for medical uses
• At home for wellness uses
• In the field for agricultural applications
• In the field for military and security applications
• In low resource environments such as the developing world

For more information

For a fabulous description of how the technology works:  https://news.vanderbilt.edu/2017/01/11/dna-duplicator-small-enough-to-hold-in-your-hand/

For the publication:  http://pubs.acs.org/doi/abs/10.1021/acs.analchem.6b03291